![SOLVED: When purifying your protein using Ni-NTA column your protocol requires elution buffer with 250mM imidazole and 15% NaCl, but you only have 3M imidazole in the lab: If you need 15mL SOLVED: When purifying your protein using Ni-NTA column your protocol requires elution buffer with 250mM imidazole and 15% NaCl, but you only have 3M imidazole in the lab: If you need 15mL](https://cdn.numerade.com/ask_images/fc3fed260f64468c99f69abfc733b327.jpg)
SOLVED: When purifying your protein using Ni-NTA column your protocol requires elution buffer with 250mM imidazole and 15% NaCl, but you only have 3M imidazole in the lab: If you need 15mL
![How can I get rid of contaminants in my protein purifications? Protein is His tagged and using Ni-NTA? | ResearchGate How can I get rid of contaminants in my protein purifications? Protein is His tagged and using Ni-NTA? | ResearchGate](https://www.researchgate.net/profile/Om-Narayan-3/post/How_can_I_get_rid_of_contaminants_in_my_protein_purifications_Protein_is_His_tagged_and_using_Ni-NTA/attachment/60ef0a672897145fbd6134e5/AS%3A1045173454766080%401626200178748/download/SDS_PAGE+Protein+purifcation.jpg)
How can I get rid of contaminants in my protein purifications? Protein is His tagged and using Ni-NTA? | ResearchGate
![Macherey-Nagel™ Protino™ Ni-TED 1000 Kit 50 prep kit of packed columns for the purification of His-Tag proteins, buffer LEW, elution buffer, protocol Macherey-Nagel Macherey-Nagel™ Protino™ Ni-TED 1000 Kit | Fisher Scientific Macherey-Nagel™ Protino™ Ni-TED 1000 Kit 50 prep kit of packed columns for the purification of His-Tag proteins, buffer LEW, elution buffer, protocol Macherey-Nagel Macherey-Nagel™ Protino™ Ni-TED 1000 Kit | Fisher Scientific](https://assets.fishersci.com/TFS-Assets/CCG/EU/Macherey-Nagel/product-images/745110_Protino_Ni-TED_1000_Packed_Column.jpg-650.jpg)
Macherey-Nagel™ Protino™ Ni-TED 1000 Kit 50 prep kit of packed columns for the purification of His-Tag proteins, buffer LEW, elution buffer, protocol Macherey-Nagel Macherey-Nagel™ Protino™ Ni-TED 1000 Kit | Fisher Scientific
![How to get rid of contaminant proteins while doing gradient elution to purify a recombinant protein having his-tag using Ni-NTA coloumn from IBs ?? | ResearchGate How to get rid of contaminant proteins while doing gradient elution to purify a recombinant protein having his-tag using Ni-NTA coloumn from IBs ?? | ResearchGate](https://www.researchgate.net/profile/Diptesh-Das/post/How_to_get_rid_of_contaminant_proteins_while_doing_gradient_elution_to_purify_a_recombinant_protein_having_his-tag_using_Ni-NTA_coloumn_from_IBs/attachment/618539a8f5675b211b0de7c3/AS%3A1086784398196740%401636121000678/download/Presentation1.jpg)